Identification of Proteins Specifically Assembled on a Stem-Loop Composed of a CAG Triplet Repeat

Human genomic DNA contains a number of diverse repetitive sequence motifs, often identified as fragile sites leading to genetic instability. Among them, expansion events occurring at triplet repeats have been extensively studied due their association with neurological disorders, including Huntington’s disease (HD). In the case HD, expanded CAG in HTT gene are thought cause onset. The is believed be triggered by emergence stem-loops composed repeats, while underlying molecular mechanisms largely unknown. Therefore, identifying proteins recruited on such stem loops would useful understand instability repeats. We previously developed plasmid pull-down methodology that captures specifically assembled any interest using nuclear extracts. Analysis Mass Spectrometry revealed among bound stem-loop many turned out belong repair pathways. expect our data set represent entry point for design assays allowing explored.